Study on the Best Surface Sterilization Method of Acacia

Abstract

Propagation of this species has been carried out mainly from seed. However, seed collected from Acacia hybrid trees is highly variable. Therefore, plant tissue culture technology has a potential to overcome this problem where it allows efficient and rapid clonal propagation of many economically important species, especially Acacia hybrid. A sterilization is one of the most important steps in tissue culture. The aim of this work was to examine the optimal kind and concentration of surface sterilization method and establish an efficient propagation system for Acacia hybrid using tissue culture technique. In this work, we studied a surface sterilization methodology and in vitro propagation of A. hybrid. In vitro propagation of the Acacia hybrid using tissue culture technique have an importance for genetic engineering work, bioenergy research and forestry industry in Thailand.